e Time course of CD68 expression at 15 and 30 days post-injury. d Micrograph at ×20 magnification of the dentate gyrus of CX3CR1 WT-TBI and CX3CR1 KO-TBI showing in the hippocampus show a strong increase in CD68 staining in the CX3CR1 KO-TBI compared to CX3CR1 WT-TBI at 30 days post-TBI. c Unbiased stereology quantification of Marco revealed a significant increase in the number of Marco + cells in the hippocampus of CX3CR1 KO-TBI compared to CX3CR1 WT-TBI ( p < 0.001). b Higher magnification (×20) of the dentate gyrus of CX3CR1 WT-TBI and CX3CR1 KO-TBI showing in the hippocampus show a strong increase in staining in the CX3CR1 KO-TBI compared to CX3CR1 WT-TBI at 30 days. a Micrograph at ×4 magnification showing Marco staining at 30 days post-TBI in the dentate gyrus of CX3CR1 WT-TBI and CX3CR1 KO-TBI mice. © 2010 Wiley‐Liss, Inc.Immunostaining and stereological quantification for M1 polarization markers in hippocampus at 30 days post-TBI.
Our data also emphasizes the importance of considering such differences when evaluating changes in adult neurogenesis in pathological conditions and following experimental procedures. Cell number estimation using a 300‐μm‐thick hypothetical slice indicates that regional differences in immature cells might contribute to the formation of topographic gradients in mature granule cells in the adult hippocampus. Taken together, these findings suggest that a larger pool of immature granule cells in dorsal hippocampus might be responsible for spatial learning and memory, whereas a smaller pool of radial glia‐like progenitors in ventral hippocampus might be associated with the susceptibility to affective disorders.
No significant regional differences were detected in the NDs of dividing cells identified by proliferating cell nuclear antigen. The NDs of calretinin‐expressing cells presumed young granule cells at the postmitotic stage were significantly higher in the suprapyramidal blade than in the infrapyramidal blade in the dorsal DG. The NDs of doublecortin‐expressing cells presumed neural progenitors and immature granule cells were significantly higher in the suprapyramidal blade of the dorsal DG than in the other subdivisions. The NDs of radial glia‐like progenitors labeled by brain lipid binding protein were significantly lower in the infrapyramidal blade of the ventral DG than in other subdivisions. The optical disector was applied to estimate the numerical densities (NDs) of labeled cells in the granule cell layer. infrapyramidal) in adult neurogenesis in the mouse hippocampus using endogenous markers. Here, we examined the topographic differences (dorsal vs. For instance, dorsal hippocampus is involved in spatial memory and learning whereas ventral hippocampus is related to emotion. Increasing evidence shows that these functions are topographically distributed along the dorsoventral (septotemporal) and transverse axes of the hippocampus. The hippocampus plays a critical role in various cognitive and affective functions. Topographic differences in adult neurogenesis in the mouse hippocampus: A stereology‐based study using endogenous markers Topographic differences in adult neurogenesis in the mouse hippocampus: A stereology‐based study.